bio rad miniprotean tetra cell apparatus (Bio-Rad)

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Bio-Rad bio rad miniprotean tetra cell apparatus
Bio Rad Miniprotean Tetra Cell Apparatus, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 2989 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/miniprotean+apparatus/Mini-PROTEAN+Tetra+Cell/pm41565204-191-11-11
Average 97 stars, based on 2989 article reviews

bio rad miniprotean tetra cell apparatus - by Bioz Stars, 2026-09

97/100 stars

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Bicinchoninic Acid Protein Assay:

Article Title: The Coordinated P53 and Estrogen Receptor Cis-Regulation at an FLT1 Promoter SNP Is Specific to Genotoxic Stress and Estrogenic Compound
Article Snippet: .. Cell extracts were quantified using the BCA protein assay kit and BSA as a reference standard (Pierce, Celbio, Milan, Italy), and equal amount of proteins were resolved on 7.5% BisTris Acrilamide gels, and transferred to Nitrocellulose or PVDF membranes (GE Healthcare, Milan, Italy) using a Biorad MiniProtean apparatus (Biorad, Milan, Italy). .. After blotting the membranes were probed with monoclonal (p53 specific: pAb1801 and DO-1, Santa Cruz Biotechnology, Milan, Italy) or polyclonal antibodies (p53: CM-1, Novocastra, Milan, Italy; p21: C-19; ERα: H-184, Santa Cruz Biotechnology, Milan, Italy).

Membrane:

Article Title: Cellular stress response, sirtuins and UCP proteins in Alzheimer disease: role of vitagenes
Article Snippet: .. The proteins were transferred onto nitrocellulose membrane (0.45 μΜ) (BIO-RAD Hercules, CA, USA) in transfer buffer containing (0.05% di SDS, 25 mM di Tris, 192 mM glycine and 20% v/v methanol) using a miniprotean apparatus (BIO-RAD). ..

Electrophoresis:

Article Title: The usefulness of lutein/trypan blue vital dye for the staining of corneal endothelium: a pilot study on DMEK pretreated tissues.
Article Snippet: .. Total protein contents were A280 quantified (NanoDrop), and normalized samples (15 μg/lane) were separated by electrophoresis on 12% SDS-polyacrylamide gel under reducing conditions (130 V/frontline; Miniprotean apparatus; Bio-Rad Laboratories, Inc., Hercules, CA, USA). .. Subsequently, the separated bands were transferred from SDS-PAGE to Hy-Bond membranes (GE Healthcare, Buckinghamshire, UK) under semi-dry conditions (10 V/55 1 3 min; semi-dry condition, transblotting apparatus; Bio-Rad) in the presence of 48 mM Tris-HCl (pH 6.8), 39 mM glycine, 0.0037% SDS, and 20% methanol solution.

Article Title: Coupling of Spectrin Repeat Modules for the Assembly of Nanorods and Presentation of Protein Domains.
Article Snippet: .. The native PAGE experiment was conducted using the miniPROTEAN apparatus (BioRad, CA, USA) with a 6 or 8% (w/v) discontinuous polyacrylamide gel (pH 8.8) in cold electrophoresis buffer (25 mM Tris−HCl pH 8.3 and 192 mM glycine) at 90 V for ∼3.5 h. The loading buffer was prepared without SDS. .. After electrophoresis, the gels were stained with InstantBlue Coomassie protein stain (Abcam Limited, Cambridge, UK) and subsequently scanned for analysis.

Article Title: Exploring the Repertoire of Rhomboid Proteases in Cryptosporidium parvum Parasite: Phylogenesis, Structural motifs and Cellular Localization in Sporozoite Cells
Article Snippet: .. Electrophoresis was performed on MiniProtean apparatus (Biorad) with precast gels 4-15% (Biorad). .. Expression of recombinant proteins was checked on 3 ml of bacterial culture induced for 3 hours with 1 mM of IPTG, pelleted and lysed with Laemmli sample buffer, loaded and ran on a 4-20% TGXTM SDS-PAGE precast gels (Bio-Rad, Hercules, CA, USA) and finally tested in Western blot as below.

Clear Native PAGE:

SDS Page:

Article Title: Alternative oxidase activity and vacuolar intrusion of mitochondria represent a delayed mitophagy associated with the chilling stress in Haberlea rhodopensis
Article Snippet: .. Solubilized proteins were separated by SDS PAGE on 10–18% gradient acrylamide gels (Laemmli, 1970 but containing 8.7% (w/V) glycerol) in a MiniProtean apparatus (BioRad) using a constant current of 20 mA per gel (surface 1 cm2) at 6 °C. .. Proteins were transferred to AmershamTM ProtranTM Premium 0.2 μm nitrocellulose blotting membranes (GE Healthcare) in 25 mM Tris (pH 8.3), 192 mM glycine, 20% (V/V) methanol and 0.02% (w/V) SDS at 6 °C using constant voltage of 90 V for 3 h. Immunoblots against mitochondrial alternative oxidase was performed with rabbit polyclonal antibody against fully conserved C-terminal consensus motifs of Arabidopsis thaliana, Solanum lycopersicum, and Oryza sativa mitochondrial alternative oxidase isoforms (AOX1/2; AS04 054; Agrisera AG, Vännäs, Sweden).

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